Files and links (1)
Preprint (Author's original)Open Access (License Unspecified), Open
Preprint
The nucleus acts as a ruler tailoring cell responses to spatial constraints
biorxiv.org
16 Oct 2020
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The microscopic environment inside a metazoan organism is highly crowded. Whether individual cells can tailor their behavior to the limited space remains unclear. In this study, we found that cells measure the degree of spatial confinement by using their largest and stiffest organelle, the nucleus. Cell confinement below a resting nucleus size deforms the nucleus, which expands and stretches its envelope. This activates signaling to the actomyosin cortex via nuclear envelope stretch-sensitive proteins, up-regulating cell contractility. We established that the tailored contractile response constitutes a nuclear ruler-based signaling pathway involved in migratory cell behaviors. Cells rely on the nuclear ruler to modulate the motive force that enables their passage through restrictive pores in complex three-dimensional environments, a process relevant to cancer cell invasion, immune responses, and embryonic development.
Metrics
22 Record Views
Details
- Title
- The nucleus acts as a ruler tailoring cell responses to spatial constraints
- Creators
- A J Lomakin - Institut CurieC J Cattin - Swiss Federal Institute of Technology in ZurichD Cuvelier - Institut Pierre-Gilles de Gennes pour la MicrofluidiqueZ Alraies - Institut CurieM Molina - King's College LondonG P F Nader - Institut Pierre-Gilles de Gennes pour la MicrofluidiqueN Srivastava - Institut Pierre-Gilles de Gennes pour la MicrofluidiqueP J Sáez - Institut Pierre-Gilles de Gennes pour la MicrofluidiqueJ M Garcia-Arcos - Institut Pierre-Gilles de Gennes pour la MicrofluidiqueI Y Zhitnyak - Russian Cancer Research Center NN BlokhinA Bhargava - Institut CurieM K Driscoll - The University of Texas Southwestern Medical CenterE S Welf - The University of Texas Southwestern Medical CenterR Fiolka - The University of Texas Southwestern Medical CenterR J Petrie - Drexel University, BiologyN S De Silva - Institut CurieJ M González-Granado - Research Institute Hospital 12 de OctubreN Manel - Institut CurieA M Lennon-Duménil - Institut CurieM Piel - Institut Pierre-Gilles de Gennes pour la MicrofluidiqueD J Müller - Swiss Federal Institute of Technology in Zurich
- Publication Details
- biorxiv.org
- Publisher
- American Physical Society (APS)
- Grant note
- K25 CA204526 / NCI NIH HHS K99 GM123221 / NIGMS NIH HHS R35 GM133522 / NIGMS NIH HHS R01 GM126054 / NIGMS NIH HHS R33 CA235254 / NCI NIH HHS
- Resource Type
- Preprint
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000579179500052
- Other Identifier
- 991021879915004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
Highly Cited Paper
- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology